Clinical Trial Finder

Procurement

Inclusion Criteria:

• - Diagnosis of GPC3-positive* solid tumors (as determined by immunohistochemistry with an extent score of >=Grade 2 [>25% positive tumor cells] and an intensity score of >= 2 [scale 0-4]).

• - Age ≥21 years.

• - Lansky or Karnofsky score ≥60% (See Appendix I) - Life expectancy ≥16 weeks.

• - Barcelona Clinic Liver Cancer Stage A, B or C (for patients with hepatocellular carcinoma only, See Appendix II) - Child-Pugh-Turcotte score <7 (for patients with hepatocellular carcinoma only, See Appendix III) - Informed consent explained to, understood by and signed by patient/guardian.

Patient/guardian given copy of informed consent * GPC3 expression will be evaluated by standard immunohistochemistry (IHC) at Texas Patients's Hospital/Baylor College of Medicine, Department of Pathology for all patients to meet procurement eligibility. All patients will send at least 5 unstained slides. Procurement

Exclusion Criteria:

• - History of hypersensitivity reactions to murine protein-containing products OR presence of human anti-mouse antibody (HAMA) prior to enrollment (only patients who have received prior therapy with murine antibodies).

• - History of organ transplantation.

• - Known HIV positivity.

• - Active bacterial, fungal or viral infection (except Hepatitis B or Hepatitis C virus infections) Treatment

  Inclusion Criteria:

  - Age ≥ 21 years.

• - Barcelona Clinic Liver Cancer Stage A, B or C (for patients with hepatocellular carcinoma only, See Appendix II) - Life expectancy of ≥ 12 weeks.

• - Lansky or Karnofsky score ≥ 60% (See Appendix I) - Child-Pugh-Turcotte score < 7 (for patients with hepatocellular carcinoma only, See Appendix III) - Adequate organ function: - Creatinine clearance as estimated by Cockcroft Gault or Schwartz ≥ 60 ml/min.

• - total bilirubin < 3 times ULN for age.

• - INR ≤1.7 (for patients with hepatocellular carcinoma only) - absolute neutrophil count > 500/µl.

• - platelet count > 25,000/µl (can be transfused) - Hgb ≥ 7.0 g/dl (can be transfused) - Pulse oximetry >90% on room air.

• - Refractory or relapsed disease after treatment with up- front therapy and at least one salvage treatment cycle.

• - Recovered from acute toxic effects of all prior chemotherapy and investigational agents before entering this study, as determined by history and physical exam.

• - Sexually active patients must be willing to utilize one of the more effective birth control methods for 3 months after the T-cell infusion.

• - Informed consent explained to, understood by and signed by patient/guardian.

Patient/guardian given copy of informed consent. Treatment

Exclusion Criteria:

• - Pregnancy or lactation.

• - Uncontrolled infection.

• - Systemic steroid treatment (greater than or equal to 0.5 mg prednisone equivalent/kg/day, dose adjustment or discontinuation of medication must occur at least 24hrs prior to CAR T cell infusion) - Known HIV positivity.

• - Active bacterial, fungal or viral infection (except Hepatitis B or Hepatitis C virus infections) - History of organ transplantation.

- History of hypersensitivity reactions to murine protein-containing products OR presence of human anti-mouse antibody (HAMA) prior to enrollment (only patients who have received prior therapy with murine antibodies)

The study team will collect up to 18 teaspoons of patients' blood. This will be used this blood to grow T cells. The study team then grows the T cells and uses a retrovirus (a special virus that can insert the GPC3 CAR and IL15 plus IL21 genes into the T cells) to genetically engineer (generate cells in the laboratory) them. After the CAR and IL15 plus IL21 genes are put into the T cells, the study team ensures that they could kill GPC3 positive solid tumor cells in the laboratory. LYMPHODEPLETION CHEMOTHERAPY: Several studies suggest that the infused T cells need room to be able to proliferate and accomplish their functions and that this may not happen if there are too many other T cells in the blood. Lymphodepletion chemotherapy is a way of temporarily decreasing the amount of the body's T cells, so that FAST-CAR T cells can grow better. with the two lymphodepleting chemotherapy drugs we will use are called cyclophosphamide (Cytoxan) and fludarabine. They will both be given daily for 3 days before receiving the T-cell infusion. WHAT THE INFUSION WILL BE LIKE: The cells will be thawed and injected into you over 5 to 10 minutes. Patients will receive the 21.15.GPC3-CAR T cells 48 to 72 hours after completing the chemotherapy. Patients may be given Tylenol (acetaminophen) and Benadryl (diphenhydramine) before the FAST-CAR T cells to prevent a possible allergic reaction.. This is a dose escalation study, which means that the study team does now know the highest dose of 21.15.GPC3-CAR T cells that is safe. To find out, the cells will be given to at least 3 participants at a lower dose. If that is safe, the study team will raise the dose level that is given to the next group of participants. The dose a patient received will get will depend on how many participants get the agent before the patient and how they react. The investigator will tell the patient this information. Since the treatment is experimental, what is likely to happen at any dose is not known. If after 4 weeks the patient's condition does not get worse, the patient may receive another round of lymphodepleting chemotherapy and infusion of 21.15.GPC3-CAR T cells. All the treatments will be given by the Center for Cell and Gene Therapy at Methodist Hospital of Houston. Medical tests before treatment: Before being treated, the patient will receive a series of standard medical tests:

• - Physical exam and history.

• - Blood tests to measure blood cells, kidney and liver function.

• - Pregnancy test (if the patient is a female who can get pregnant) - If the patient is infected with the hepatitis B virus (HBV) sthe study team will do a test to measure the levels of the virus.

• - Measurements of the patient's tumor by scans (either CT or MRI).

Side effects are rare and include risk of developing an unrelated cancer due to radiation exposure from the machine, but this is unlikely.

• - the tumor marker alfa-fetoprotein (AFP), if the patient's tumor produces this protein.

Medical tests during and after treatment: The patient will receive standard medical tests when you are getting the infusions and after:

• - Physical exams and History.

• - Blood tests to measure blood cells, kidney and liver function.

• - If the patient is infected with the hepatitis B virus (HBV) the study team will repeat the test and monitor the levels of the virus.

• - Measurements of the patient's tumor by scans (same scans used before treatment) (4 - 6 weeks after the infusion) and AFP (if applicable at 1, 2, and 4 weeks after the infusion).

• - Tumor biopsy of an accessible tumor between 2-4 weeks after the infusion and as clinically indicated thereafter.

For additional clinically indicated tumor biopsies the study team will ask for a portion of the sample for research. The study team will not obtain biopsies if the patient's tumors are not accessible. FOLLOW-UP STUDIES: The study team will follow the patient during and after the injections. To learn more about the way the T cells are working in the patient's body, up to 60 mL (up to 12 teaspoons) of blood will be taken from the patient before the chemotherapy, before the T-cell infusion, 1 to 4 hours after the infusion, 3 to 4 days after the infusion (this time point is optional ) at 1 week, 2 weeks, 4 weeks and 8 weeks after the injection, every 3 months for 1 year, every 6 months for 4 years and then every year for the next 10 years. Total participation time for this study will be 15 years. During the time points listed above, if the T cells are found in the patient's blood at a certain amount an extra 5mL of blood may need to be collected for additional testing . The study team will use this blood to look for the frequency and activity of the cells that we have given; that is, to learn more about the way the T cells are working and how long they last in the body. The study team will also use this blood to see if there are any long-term side effects of putting the new gene (chimeric antigen receptor, CAR) into the cells. In addition to the blood draws, because the patient will receive cells that have had a new gene put in them, the patient will need to have long term follow up for 15 years so the study team can see if there are any long-term side effects of the gene transfer. Once a year, the patient will be asked to have blood drawn and answer questions about their general health and medical condition. The investigators may ask the patient to report any recent hospitalizations, new medications, or the development of conditions or illness that were not present when the patient enrolled in the study and may request that physical exams and/or laboratory tests be performed if necessary. When tumor biopsy is performed for clinical reasons the study team will request permission to obtain excess sample to learn more about the effects of the treatment on the patient's disease. In the event of death, the study team will request permission to perform an autopsy (a procedure where a doctor examines the body to look for a cause of death) to learn more about the effects of the treatment on the patient's disease and if there were any side effects from the cells with the new gene. In addition, the study team will ask for your permission to use tumor biopsy for research purposes only. Associated risk with the biopsy will be discussed with the patient in detail in a procedure specific consent form. The study team will test the sample to see if the 21.15.GPC3-CAR T cells can be found in the tumor and what effect they had on the tumor cells. If the patient develops a second abnormal cancer growth, significant blood or nervous system disorder during the trial, the study team will request that a biopsy sample of the tissue will be tested. The remaining blood and/or tissue samples that are not needed directly for the patient could be used to help researchers learn about this disease and/or immune therapy. These specimens and information about the patient's circumstances may be shared with other cancer researchers. Although there will be a record identifying under what circumstances these specimens were obtained, under all circumstances the patient's identity will be kept confidential. There is a small risk for the loss of confidentiality. However, study personnel will make every effort to minimize this risk. Samples will be kept at Baylor College of Medicine until they are exhausted.

Arms

Experimental: 21.15.GPC3-CAR T cells

GPC3-CAR and the IL15 plus IL21 will be administered to patients with GPC3-positive solid tumors.

Interventions

Genetic: - 21.15.GPC3-CAR T cells

Three different dosing schedules will be evaluated. The following dose levels will be evaluated: The following dose levels will be evaluated: DL0: 3x10^7/m2 DL1: 1x10^8/m2 DL2: 3x10^8/m2 DL3: 1x10^9/m2